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Test paper for dental students - Virology test (2005) Part 5

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Question Answer
What is PCR?
What are three methods to use in growth and assay of viruses?
What is the plaque assay used for?
T or F: All viruses produce Cytopathic effect (CPE)
What definition: The characteristic morphological changes which occur in the cell due to the infection of a particular virus.
T or F: Growth and assay of viruses requires a high multiplicity of infection (MOI)
The reproductive cycles of ALL viruses exhibit what common feature?
What is the singlemost important thing produced by viruses that gives them the ability to multiply and determines the fate of the infected cell?
What type of infection: The infection and subsequent lysis of susceptible cells by a virus.
What type of infection: The abnormal growth of cells resulting from the continuous expression of one or more viral genes.
What type of infection: The virus persists in the cell in a noninfectious form.
What type of infection: The infection of a cell which does NOT result in the multiplication of infectious viral progeny.
What type of infection: The infection of a receptive cell with a virus particle which results in the MULTIPLICATION of infectious viral progeny.
Name the 5 types of Infections:
What determines host range? (2 things)
T or F: Sometimes the target cells are at the portal of entry.
What are the cells called that are targeted by the virus and result in clinical disease?
Cells that become immediately infected are susceptible cells at the ______ ___ __________.
What definition? The capacity of a cell or animal to become infected.
What definition? The spectrum of cells which can be productively infected by a given virus.
What definition? The kinds of tissue cells and animal species that a virus can productively infect.
Steps to multiply a virus
No clearly identifiable capsid. But several coats around the nucleic acid. An example is _______
Enveloped virus w/ helical symmetry, the _____ is coiled w/in the ________. An example is ______
Enveloped virus w/ icosahedral symmetry, the _____ is surrounded by the ________. An example is ______
Morphological subunits that can be seen when capsid is broken (hard to "see" in an intact virus). Surface structures composed of 5-6 protein molecules
Symmetry observed w/ spherically shaped viruses. Involves the packing together of many identical subunits. (soccer ball)
Can helical particles form w/o the genome?
Helical symmetry examples (5)
The capsid of a virus composed of many copies of a single kind of protein subunit arranged in a close-packed helix
The simplest viral symmetry (proteins bind to nucleic acid not a capsule)
The symmetry observed w/ cylindrical viruses (like a slinky)
The structure of the capsid &/or envelope (spikes) has important implications for (3 things)
Virion structural protein which locates on the cytoplasmic side of the transmem glycoprotein spike. It attracts the completed nucleocapsid for mem fusion.
Protein or glycoprotein structure(s) which emanates from the surface of the virus particle
The lipid bilayer & associated glycoproteins that surround many types of virus particles
The capsid & nucleocapsid are composed of ___ or at most ___ different kinds of proteins in a crystalline array. Why?
The complete protein-nucleic acid complex that is the packaged from of the genome in a virus particle
The protein shell directly surrounding the viral nucleic acid.
The association of core proteins & the genome is required to _______ the viral genome during viral ______ & virion _______
The _____ is the association between nucleic acid & basic proteins either coded by the virus or histones from the host cell
Retroviruses have a ______ genome
The viral genome can have 3 basic morphologies
The viral genome can be ______ or ______ stranded
The ability of viruses to pass through ______ is used to ID an unknown infectious agent as a bact or virus.
The largest virus
The smallest virus
viruses are _____-_____ times smaller then the cells they infect
A virus is an __________ parasite
One (complete) virus particle
A virus is made up of ________ & _________
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